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KMID : 0381120120340030339
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Genes and Genomics
2012 Volume.34 No. 3 p.339 ~ p.343
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Novel HMW glutenin genes from Aegilops tauschii and their unique structures
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Chen Wen-Jie
Yuan Zhong-Wei
Zhang Lian-Quan
Fan Xing
Yan Ze-Hong
Wang Ji-Rui
Zheng You-Liang
Zhang Huai-Gang
Liu Deng-Cai
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Abstract
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A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx5.3t and 1Dy12.1**t were revealed and characterized from Ae. tauschii accession PI554324. SDS-PAGE band of 1Dx5.3t was between those of 1Bx6 and 1Bx7, while 1Dy12.1**t with slightly faster migration rate than that of 1Dy12. The lengths of 1Dx5.3 t and 1Dy12.1** t were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues, respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. 1Dx5.3t is the shortest of the known Dx-type alleles. 1Dy12.1**t is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. This cysteine that is not existed in previously reported Dy-type genes may be useful for improving bread wheat quality. Median-joining Network analysis indicated that 1Dy12.1** t may be a key site in the genealogy of the Glu-Dy.
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KEYWORD
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Aegilops tauschii, Bacterial expression, HMW-GS, Phylogenetic analysis
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